Developmental Potential of Nuclear-transferred Embryos Using a Bovine Mammary Epithelial Cell Line (bmec)
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O-3: Effect of Melatonin Treatment on Developmental Potential of Somatic Cell Nuclear- Transferred Mouse Oocytes In Vitro
Background Melatonin (N-acetyl-5- methoxytryptamine) is mainly synthesized and secreted in the pineal gland, ovary, testes, bone marrow, retina and lens in mammalian species. It is involved in the detoxification of ROS and protects embryos from oxidative damage. Melatonin acts as a potential free radical scavenger, including peroxyl radical and hydroxyl radical. In addition, it can stimulate th...
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Escherichia coli is a frequent cause of clinical mastitis in dairy cows. It has been shown that a prompt response of the mammary gland after E. coli entry into the lumen of the gland is required to control the infection, which means that the early detection of bacteria is of prime importance. Yet, apart from lipopolysaccharide (LPS), little is known of the bacterial components which are detecte...
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Background: Development of cloned bovine embryos. Materials and Methods: Oocytes collection,oocyte denudation, oocyte enucleation, nuclear transfer, electrofusion, reconstructed embryo activation, culture of reconstructed and IVF embryo,and treatment with SAH post fusion interval Treatment of reconstructed embryos with TSA for 12 hours after activation, preparation of somatic donor cells, donor...
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Background: The beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on the mouse somatic cell nuclear transfer remained unknown. Materials and Methods: In this study, we assessed the effects of various concentrations of melatonin (10-6 to 10-12 M) on t...
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Background: Staphylococci are recognized worldwide as one of the most important etiological agents of bovine mastitis due to their virulence factors such as their ability to penetrate inside mammary epithelial cells and their ability to form biofilm. Aims: The objectives of this study were to establish a model of primary mammary epithelial cells origin...
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تاریخ انتشار 2001